Padina arborescensextract protects high glucose-induced apoptosis in pancreatic β cells by reducing oxidative stress

Padina arborescens extract protects high glucose-induced apoptosis in pancreatic β cells by reducing oxidative stress

BACKGROUND/OBJECTIVES This study investigated whether Padina arborescens extract (PAE) protects INS-1 pancreatic β cells against glucotoxicity-induced apoptosis. MATERIALS/METHODS Assays, including cell viability, lipid peroxidation, generation of intracellular ROS, NO production, antioxidant enzyme activity and insulin secretion, were conducted. The expressions of Bax, Bcl-2, and caspase-3 p...

Berberine protects against glutamate-induced oxidative stress and apoptosis in PC12 and N2a cells

Objective(s): Neurodegenerative diseases have been associated with glutamatergic dysfunction. Berberine, an isoquinoline alkaloid broadly present in different medicinal herbs, has been reported to have neuroprotective effect. In the present study, the effects of berberine against glutamate-induced oxidative damage and apoptosis were investigated. Materials and Methods: The cultured PC12 and N2a...

Bach1 deficiency protects pancreatic β-cells from oxidative stress

Bach1 deficiency protects pancreatic β-cells from oxidative stress injury.

BTB and CNC homology 1 (Bach1) is a transcriptional repressor of antioxidative enzymes, such as heme oxygenase-1 (HO-1). Oxidative stress is reportedly involved in insulin secretion impairment and obesity-associated insulin resistance. However, the role of Bach1 in the development of diabetes is unclear. HO-1 expression in the liver, white adipose tissue, and pancreatic islets was markedly upre...

Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress

Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether...